A. Garnier

Latest

• Overview of current scalable methods for purification of viral vectors.
• New protocol for lentiviral vector mass production.
• Identification of host proteins associated with retroviral vector particles by proteomic analysis of highly purified vector preparations
• Purification of retrovirus particles using heparin affinity chromatography
• A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus
• Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography
• Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification
• Downstream processing of oncoretroviral and lentiviral gene therapy vectors
• Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation
• Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor
• A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography
• On-line measurement of green fluorescent protein (GFP) fluorescence for the monitoring of recombinant adenovirus production
• Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells
• Study of adenovirus production in serum-free 293SF suspension culture by GFP-expression monitoring
• Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells
• Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies
• Determination of mass transfer parameters in surface aerated bioreactors with bubble entrainment
• Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells