B. Massie

Latest

• Establishment and validation of new complementing cells for production of E1-deleted adenovirus vectors in serum-free suspension culture
• Dystrophin expression in muscle following gene transfer with a fully deleted ("Gutted") adenovirus is markedly improved by trans-acting adenoviral gene products
• Adenovirus-mediated utrophin gene transfer mitigates the dystrophic phenotype of mdx mouse muscles
• Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells
• Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies
• Use of the centritech lab centrifuge for perfusion culture of hybridoma cells in protein-free medium
• Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection
• Scale-up of recombinant virus and protein production in stirred-tank reactors.
• Baculovirus expression system scaleup by perfusion of high‐density Sf‐9 cell cultures
• Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures
• Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells
• Culture of insect cells in helical ribbon impeller bioreactor