R. Tom

Latest

• Reassessing culture media and critical metabolites that affect adenovirus production
• Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions
• On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements
• Identification and crystallization of a protease-resistant core of calnexin that retains biological activity
• Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells
• On-line monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures
• Insect cell density in bioreactor cultures can be estimated from on-line measurements of optical density
• Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures
• Growth, Nutrient Consumption, and End‐Product Accumulation in Sf‐9 and BTI‐EAA Insect Cell Cultures: Insights into Growth Limitation and Metabolism