S. Perret

Latest

• Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions
• Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis
• Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency
• High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.
• A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture
• Fed-batch culture of Sf-9 cells supports 3 x 107 cells per ml and improves baculovirus-expressed recombinant protein yields
• Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells
• On-line monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures
• Insect cell density in bioreactor cultures can be estimated from on-line measurements of optical density