A.A. Kamen

Latest

• Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors via Fedbatch in an Insect Cell-One Baculovirus System
• Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics
• Large-scale transient transfection of suspension mammalian cells for VLP production
• RNA interference technology to improve the baculovirus-insect cell expression system
• Endothelial IL-33 expression is augmented by adenoviral activation of the DNA damage machinery
• Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza
• Production of virus-like particles for vaccination
• Rational plasmid design and bioprocess optimization to enhance recombinant adeno-associated virus (AAV) productivity in mammalian cells
• Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system
• Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems
• Critical review of current and emerging quantification methods for the development of influenza vaccine candidates
• Current and emerging cell culture manufacturing technologies for influenza vaccines
• Large-scale adenovirus and poxvirus-vectored vaccine manufacturing to enable clinical trials
• Particle quantification of influenza viruses by high performance liquid chromatography
• Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
• Analytical technologies for influenza virus-like particle candidate vaccines: Challenges and emerging approaches
• An initiative to manufacture and characterize baculovirus reference material
• Development and validation of a HPLC method for the quantification of baculovirus particles
• Insect cell technology is a versatile and robust vaccine manufacturing platform
• Manufacturing of adenovirus vectors: production and purification of helper dependent adenovirus.
• Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture
• Overview of current scalable methods for purification of viral vectors.
• Bioprocessing of baculovirus vectors: A review
• Improving adeno-associated vector yield in high density insect cell cultures
• Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing
• Development of a simple and high-yielding fed-batch process for the production of influenza vaccines
• Critical assessment of current adeno-associated viral vector production and quantification methods
• Improving AAV vector yield in insect cells by modulating the temperature after infection
• Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells
• Virus-like particle and viral vector production using the baculovirus expression vector system/ insect cell system: Adeno-associated virus-based products
• Production of adeno-associated viral vectors in insect cells using triple infection: Optimization of baculovirus concentration ratios
• Production of recombinant adeno-associated viral vectors using a baculovirus/insect cell suspension culture system: From shake flasks to a 20-L bioreactor
• Increased production of active human β<inf>2</inf>-adrenergic/G<inf>αs</inf> fusion receptor in Sf-9 cells using nutrient limiting conditions
• Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 107 cells per mL and production of β-galactosidase at high cell density by fed batch culture
• On-line monitoring of physiological parameters of insect cell cultures during the growth and infection process
• Understanding factors that limit the productivity of suspension-based perfusion cultures operated at high medium renewal rates
• On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements
• Identification and crystallization of a protease-resistant core of calnexin that retains biological activity
• A flow-injection (FI) mediated biosensor for on-line monitoring of lactate in mammalian cell culture
• Fed-batch culture of Sf-9 cells supports 3 x 107 cells per ml and improves baculovirus-expressed recombinant protein yields
• On-line monitoring of glucose in mammalian cell culture using a flow injection analysis (FIA mediated biosensor
• On-line monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures
• Use of the centritech lab centrifuge for perfusion culture of hybridoma cells in protein-free medium
• Determination of mass transfer parameters in surface aerated bioreactors with bubble entrainment
• Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection
• Scale-up of recombinant virus and protein production in stirred-tank reactors.
• Baculovirus expression system scaleup by perfusion of high‐density Sf‐9 cell cultures
• Protein-free culture medium improvement: testing additives and their interactive effects in 96-well plates
• Design parameters and performance of a surface baffled helical ribbon impeller bioreactor for the culture of shear sensitive cells
• Culture of insect cells in helical ribbon impeller bioreactor