Y. Durocher

Latest

• Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza
• Cell Transfection
• Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII
• New protocol for lentiviral vector mass production.
• An efficient and scalable process for helper-dependent adenoviral vector production using polyethylenimine-adenofection
• Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures
• Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions
• Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells
• Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification
• Scalable serum-free production of recombinant adeno-associated virus type 2 by transfection of 293 suspension cells
• Large-scale transfection of mammalian cells for the fast production of recombinant protein
• Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme
• Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis
• Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme
• Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency
• High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.
• Increased production of active human β<inf>2</inf>-adrenergic/G<inf>αs</inf> fusion receptor in Sf-9 cells using nutrient limiting conditions
• A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture