Project

Genome-wide CRISPR screen to identify genes that increase the yield and functionality of AAV vectors.

Skills

• Cell culture techniques, Host-virus interaction- signaling pathway and actin regulation.
• Lentivirus production and transduction, stable cell line generation by overexpression.
• Microscopy – TIRF, Confocal microscopy.
• Biochemical assays: membrane flotation assay, subcellular fractionation, immunofluorescence, mini replicon nanoluciferase assay, immunoprecipitation, GST pull down assay.

Work experience

• May 2018 – Dec. 2019: Postdoctoral Researcher at Dr. Ronald Harty Lab, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, USA.