Publications | Viral Vectors and Vaccines Bioprocessing Group

Monitoring the Recombinant Adeno-Associated Virus Production using Extended Kalman Filter

The recombinant adeno-associated virus (rAAV) is a viral vector technology for gene therapy that is considered the safest and most …

Cristovão Freitas Iglesias, Xingge Xu, Varun Mehta, Mounia Akassou, Alina Venereo Sanchez, Nabil Belacel, Professor Amine Kamen, Miodrag Bolic

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Bioprocessing of Viral Vaccines

This book focuses on cell culture-produced viral vaccines to meet the needs of the rapidly expanding research and development in …

Professor Amine Kamen, Laura Cervera

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From functional genomics of vero cells to CRISPR-based genomic deletion for improved viral production rates

Despite their wide use in the vaccine manufacturing field for over 40 years, one of the main limitations to recent efforts to develop …

Marie-Angélique SENE, Yu Xia, Professor Amine Kamen

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Genomics of vero cells: understanding this cell line and its virus-host interactions for improved vaccine production

Marie-Angélique SENE, Sascha Kiesslich, Haig Djambazian, Ioannis Ragoussis, Yu Xia, Professor Amine Kamen

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Suspension Vero cell culture technology for high titer production of viral vaccines

Vero cells are considered as the most widely accepted continuous cell line by the regulatory authorities (such as WHO) for the …

Chun Fang Shen, Renald Gilbert, Elodie Burney, Seyyed Mehdy Elahi, Stephane Lanthier, Professor Amine Kamen, Martin Loignon

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Optimization of rAd5 vectored Newcastle vaccine production in HEK293 at high cell densities

Newcastle disease (ND) is a highly contagious and often severe global spread syndrome that affects birds including domestic poultry. It …

Samia Rourou, Amani Chaaben, Alya Soudani, Meriem Ben Zakour, Omar Farnos, Esayas Gelaye, Martha Yami, Professor Amine Kamen, Khaled Trabelsi, Héla Kallel

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Multivalent Influenza vaccine production in HEK-293 cells in response to pandemic threats

Influenza virus infects millions of people every year worldwide, with elderly and very young people among the most critically affected. …

Professor Amine Kamen, Cécile Toussaint, Omar Farnos, David Sharon, Alina Venereo Sanchez, Irene González Domínguez, Alice Bernier, Xingge Xu, Hanan Chaabane

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Intensification of influenza virus production in fed-batch and perfusion cultures of HEK293SF cells

More than half a million people die every year from complications of seasonal influenza, and vaccination stands as the most effective …

Cristina Silva, Professor Amine Kamen, Olivier Henry

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Development of scalable downstream processing platform for HEK293SF cell-based influenza vaccine production

More than half a million people die every year from complications of seasonal influenza, and vaccination stands as the most effective …

Professor Amine Kamen, Xingge Xu, Zeyu Yang, Irene González Domínguez, Cécile Toussaint, Alice Bernier, Alina Venereo Sanchez, Omar Farnos, Cristina Silva

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An adenovirus-based vaccine manufacturing technology platform for mucosal or parenteral immunization against poultry diseases in sub-Saharan Africa

Developing novel vaccine technology platforms to respond to emergency situations such as pandemic threats or zoonotic diseases is a …

Omar Farnos, Esayas Gelaye, Samia Rourou, Alya Soudani, Khaled Trabelsi, Alice Bernier, Kumar Subramaniam, Martha Yami, Professor Amine Kamen

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Development and Scalable Production of Newcastle Disease Virus-Vectored Vaccines for Human and Veterinary Use

The COVID-19 pandemic has highlighted the need for efficient vaccine platforms that can rapidly be developed and manufactured on a …

Julia Fulber, Professor Amine Kamen

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Overview of recent advances in Vero cells genomic characterization and engineering for high-throughput vaccine manufacturing

Abstract Background The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 30 years of …

Marie-Angélique SENE, Yu Xia, Professor Amine Kamen

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Haplotype-resolved de novo assembly of the Vero cell line genome

The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 40 years of accumulated …

Marie-Angélique Sène, Sascha Kiesslich, Haig Djambazian, Jiannis Ragoussis, Yu Xia, Amine A Kamen

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Critical Assessment of Purification and Analytical Technologies for Enveloped Viral Vector and Vaccine Processing and Their Current Limitations in Resolving Co-Expressed Extracellular Vesicles

Viral vectors and viral vaccines are invaluable tools in prevention and treatment of diseases. Many infectious diseases are controlled …

Aline Do Minh, Amine A. Kamen

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Vectored-Vaccine Platforms Enabled Rapid Development of Safe and Effective Vaccines in Response to COVID-19 Pandemic Situation

In 2019, an outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease …

Amine A. Kamen

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Development and Validation of an Anion Exchange High-Performance Liquid Chromatography Method for Analysis of Empty Capsids and Capsids Encapsidating Genetic Material in a Purified Preparation of Recombinant Adeno-Associated Virus Serotype 5

The development of various manufacturing platforms and analytical technologies has substantially contributed to successfully …

Pranav R.H. Joshi, Alice Bernier, Parminder S. Chahal, Professor Amine Kamen

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Recent advances and current challenges in process intensification of cell culture-based influenza virus vaccine manufacturing

Abstract Every year, millions of people are infected by the influenza virus around the world, which results in more than half a million …

Cristina A. T. Silva, Amine A. Kamen, Olivier Henry

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Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures

Lentiviral vectors (LVs) are a powerful tool for gene and cell therapy and human embryonic kidney cells (HEK293) have been extensively …

Aline Do Minh, Alexandra T. Star, Jacek Stupak, Kelly M. Fulton, Arsalan S. Haqqani, Jean-François Gélinas, Jianjun Li, Susan M. Twine, Amine A. Kamen

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Bioreactor production of rVSV-based vectors in Vero cell suspension cultures

The Vero cell line is the most used continuous cell line in viral vaccine manufacturing. This adherent cell culture platform requires …

Sascha Kiesslich, Gyoung N. Kim, Chun F. Shen, C. Yong Kang, Amine A. Kamen

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Cross-Validation of ELISA and a Portable Surface Plasmon Resonance Instrument for IgG Antibodies Serology with SARS-CoV-2 Positive Individuals

Abdelhadi Djaileb, Maryam Hojjat Jodaylami, Julien Coutu, Pierre Ricard, Mathieu Lamarre, lea rochet, Stella Cellier-Goetghebeur, devin macauley, Benjamin Charron, Vincent Thibault, Keisean Stevenson, Simon Forest, Ludovic S. Live, Nanouk Abonnenc, Anthony Guedon, Patrik Quessy, Jean-Francois Lemay, Omar Farnos, Amine A. Kamen, Matthew Stuible, Christian Gervais, Yves Durocher, François Cholette, Christine Mesa, John Kim, Marie-Pierre Cayer, Marie-Joëlle De Grandmont, Danny Brouard, Sylvie Trottier, Denis Boudreau, Joelle N. Pelletier, Jean-Francois Masson

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Development of a scalable and robust AEX method for enriched rAAV preparations in genome-containing VCs of serotypes 5, 6, 8, and 9

Removal of empty capsids from adeno-associated virus (AAV) manufacturing lots remains a critical step in the downstream processing of …

Pranav R. H. Joshi, Alice Bernier, Pablo D. Moço, Joseph Schrag, Parminder S. Chahal, Professor Amine Kamen

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Advancements in molecular design and bioprocessing of recombinant adeno-associated virus gene delivery vectors using the insect-cell baculovirus expression platform

Abstract Despite rapid progress in the field, scalable high-yield production of adeno-associated virus (AAV) is still one of the …

Pranav R.H. Joshi, Alina Venereo Sanchez, Parminder S. Chahal, Amine A. Kamen

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Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation

Vaccine design strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are focused on the Spike protein or its …

Omar Farnós, Alina Venereo-Sánchez, Xingge Xu, Cindy Chan, Shantoshini Dash, Hanan Chaabane, Janelle Sauvageau, Fouad Brahimi, Uri Saragovi, Denis Leclerc, Amine A. Kamen

Evaluation of novel HIV vaccine candidates using recombinant vesicular stomatitis virus vector produced in serum-free Vero cell cultures

Acquired Immune Deficiency Syndrome (AIDS) in humans is a result of the destruction of the immune system caused by Human …

Mathias Mangion, Jean-François Gélinas, Anahita Bakhshi Zadeh Gashti, Hiva Azizi, Sascha Kiesslich, Nasha Nassoury, Parminder S Chahal, Gary Kobinger, Rénald Gilbert, Alain Garnier, Bruno Gaillet, Professor Amine Kamen

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Vero cell upstream bioprocess development for the production of viral vectors and vaccines

The Vero cell line is considered the most used continuous cell line for the production of viral vectors and vaccines. Historically, it …

Sascha Kiesslich, Amine A. Kamen

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A pooled genome-wide screening strategy to identify and rank influenza host restriction factors in cell-based vaccine production platforms

Cell-derived influenza vaccines provide better protection and a host of other advantages compared to the egg-derived vaccines that …

David M. Sharon, Sean Nesdoly, Hsin J. Yang, Jean-François Gélinas, Yu Xia, Sven Ansorge, Amine A. Kamen

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Establishing a Robust Manufacturing Platform for Recombinant Veterinary Vaccines: An Adenovirus-Vector Vaccine to Control Newcastle Disease Virus Infections of Poultry in Sub-Saharan Africa

Developing vaccine technology platforms to respond to pandemic threats or zoonotic diseases is a worldwide high priority. The risk of …

Omar Farnós, Esayas Gelaye, Khaled Trabelsi, Alice Bernier, Kumar Subramani, Héla Kallel, Martha Yami, Amine A. Kamen

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Titration methods for rVSV-based vaccine manufacturing

The recombinant Vesicular Stomatitis Virus (rVSV) is an emerging platform for viral vector-based vaccines. Promising results have been …

Jean-François Gélinas, Sascha Kiesslich, Rénald Gilbert, Amine A. Kamen

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Serum-free production of rVSV-ZEBOV in Vero cells: microcarrier bioreactor versus scale-X™ hydro fixed-bed

Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in …

Sascha Kiesslich, José Pedro Vila-Chã Losa, Jean-François Gélinas, Amine A. Kamen

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Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture …

Aline Do Minh, Michelle Yen Tran, Amine A. Kamen

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Adeno-Associated Viral Vectors for Homology-Directed Generation of CAR-T cells

Immunotherapy with T cells expressing chimeric antigen receptors (CAR) is an emerging and promising treatment against refractory …

Pablo D. Moço, Noga Aharony, Professor Amine Kamen

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Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells

Ebola virus disease is an urgent international priority. Promising results for several vaccine candidates have been reported in …

Jean-François Gélinas, Hiva Azizi, Sascha Kiesslich, Stéphane Lanthier, Jannie Perdersen, Parminder S. Chahal, Sven Ansorge, Gary Kobinger, Rénald Gilbert, Amine A. Kamen

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Extended gene expression for Gag VLP production achieved at bioreactor scale

J. Fuenmayor, L. Cervera, F. Gòdia, A. Kamen

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Development of suspension adapted Vero cell culture process technology for production of viral vaccines

Vero cells are considered as the most widely accepted continuous cell line by the regulatory authorities (such as WHO) for the …

Chun Fang Shen, Claire Guilbault, Xiuling Li, S. Mehdy Elahi, Sven Ansorge, Professor Amine Kamen, Rénald Gilbert

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Characterization of influenza H1N1 Gag virus-like particles and extracellular vesicles co-produced in HEK-293SF

One of the concerns associated with the use of influenza virus-like particles (VLPs) as vaccine candidate or delivery system is their …

Alina Venereo-Sánchez, Kelly Fulton, Krisztina Koczka, Susan Twine, Parminder Chahal, Sven Ansorge, Rénald Gilbert, Olivier Henry, Professor Amine Kamen

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Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors via Fedbatch in an Insect Cell-One Baculovirus System

P.R.H. Joshi, L. Cervera, I. Ahmed, O. Kondratov, S. Zolotukhin, J. Schrag, P.S. Chahal, A.A. Kamen

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Process intensification for high yield production of influenza H1N1 Gag virus-like particles using an inducible HEK-293 stable cell line

A. Venereo-Sanchez, M. Simoneau, S. Lanthier, P. Chahal, L. Bourget, S. Ansorge, R. Gilbert, O. Henry, A. Kamen

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Rational plasmid design and bioprocess optimization to enhance recombinant adeno-associated virus (AAV) productivity in mammalian cells

V.V. Emmerling, A. Pegel, E.G. Milian, A. Venereo-Sanchez, M. Kunz, J. Wegele, A.A. Kamen, S. Kochanek, M. Hoerer

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Production of virus-like particles for vaccination

C.M. Thompson, M.G. Aucoin, A.A. Kamen

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Optimization and scale-up of cell culture and purification processes for production of an adenovirus-vectored tuberculosis vaccine candidate

C.F. Shen, D. Jacob, T. Zhu, A. Bernier, Z. Shao, X. Yu, M. Patel, S. Lanthier, A. Kamen

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Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate

A. Venereo-Sanchez, R. Gilbert, M. Simoneau, A. Caron, P. Chahal, W. Chen, S. Ansorge, X. Li, O. Henry, A. Kamen

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Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery

P.S. Chahal, E. Schulze, R. Tran, J. Montes, A.A. Kamen

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Evaluation of homogeneity and genetic stability of REOLYSIN® (pelareorep) by complete genome sequencing of reovirus after large scale production

R. Chakrabarty, H. Tran, Y. Fortin, Z. Yu, S.-H. Shen, J. Kolman, D. Onions, R. Voyer, A. Hagerman, S. Serl, A. Kamen, B. Thompson, M. Coffey

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Establishment and validation of new complementing cells for production of E1-deleted adenovirus vectors in serum-free suspension culture

R. Gilbert, C. Guilbault, D. Gagnon, A. Bernier, L. Bourget, S.M. Elahi, A. Kamen, B. Massie

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Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase, improves adenovirus production

C.F. Shen, A. Kamen

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A novel polyethyleneimine-coated adenoassociated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: Preparation and in vitro analysis

W. Shao, A. Paul, S. Abbasi, P.S. Chahal, J.A. Mena, J. Montes, A. Kamen, S. Prakash

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An efficient process for the purification of helper-dependent adenoviral vector and removal of helper virus by iodixanol ultracentrifugation

E. Dormond, P. Chahal, A. Bernier, R. Tran, M. Perrier, A. Kamen

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Probing inhibitory effects of destruxins from Metarhizium anisopliae using insect cell based impedance spectroscopy: Inhibition vs chemical structure

K.B. Male, Y.-M. Tzeng, J. Montes, B.-L. Liu, W.-C. Liao, A. Kamen, J.H.T. Luong

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Identification of critical infection parameters to control helper-dependent adenoviral vector production

E. Dormond, M. Perrier, A. Kamen

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From the first to the third generation adenoviral vector: What parameters are governing the production yield?

E. Dormond, M. Perrier, A. Kamen

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Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media

K. Ghani, X. Wang, P.O. De Campos-Lima, M. Olszewska, A. Kamen, I. Rivière, M. Caruso

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Development of a simple and high-yielding fed-batch process for the production of influenza vaccines

J. Meghrous, W. Mahmoud, D. Jacob, R. Chubet, M. Cox, A.A. Kamen

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Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

S. Ansorge, S. Lanthier, J. Transfiguracion, Y. Durocher, O. Henry, A. Kamen

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An efficient and scalable process for helper-dependent adenoviral vector production using polyethylenimine-adenofection

E. Dormond, A. Meneses-Acosta, D. Jacob, Y. Durocher, R. Gilbert, M. Perrier, A. Kamen

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Rapid and reliable quantification of reovirus type 3 by high performance liquid chromatography during manufacturing of Reolysin®

J. Transfiguracion, A. Bernier, R. Voyer, H. Coelho, M. Coffey, A. Kamen

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Purification of retrovirus particles using heparin affinity chromatography

M. De Las Mercedes Segura, A. Kamen, A. Garnier

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Probing inhibitory effects of Antrodia camphorata isolates using insect cell-based impedance spectroscopy: Inhibition vs chemical structure

K.B. Male, Y.K. Rao, Y.-M. Tzeng, J. Montes, A. Kamen, J.H.T. Luong

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Identification of host proteins associated with retroviral vector particles by proteomic analysis of highly purified vector preparations

M.M. Segura, A. Garnier, M.R. Di Falco, G. Whissell, A. Meneses-Acosta, N. Arcand, A. Kamen

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Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions

A. Meneses-Acosta, E. Dormond, D. Jacob, R. Tom, A. Bernier, S. Perret, G. St-Laurent, Y. Durocher, R. Gilbert, A. Kamen

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Critical assessment of current adeno-associated viral vector production and quantification methods

M.G. Aucoin, M. Perrier, A.A. Kamen

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Virus-like particle and viral vector production using the baculovirus expression vector system/ insect cell system: Adeno-associated virus-based products

M.G. Aucoin, D. Jacob, P.S. Chahal, J. Meghrous, A. Bernier, A.A. Kamen

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Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification

M.M. Segura, A. Garnier, Y. Durocher, H. Coelho, A. Kamen

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Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system

P.S. Chahal, M.G. Aucoin, A. Kamen

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Generation of a high-titer packaging cell line for the production of retroviral vectors in suspension and serum-free media

K. Ghani, S. Cottin, A. Kamen, M. Caruso

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Use of cell bleed in a high cell density perfusion culture and multivariable control of biomass and metabolite concentrations

J.-S. Deschênes, A. Desbiens, M. Perrier, A. Kamen

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Production of adeno-associated viral vectors in insect cells using triple infection: Optimization of baculovirus concentration ratios

M.G. Aucoin, M. Perrier, A.A. Kamen

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Production of recombinant adeno-associated viral vectors using a baculovirus/insect cell suspension culture system: From shake flasks to a 20-L bioreactor

J. Meghrous, M.G. Aucoin, D. Jacob, P.S. Chahal, N. Arcand, A.A. Kamen

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Production and formulation of adenovirus vectors

N.E. Altaras, J.G. Aunins, R.K. Evans, A. Kamen, J.O. Konz, J.J. Wolf

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Metabolic flux analysis of HEK-293 cells in perfusion cultures for the production of adenoviral vectors

O. Henry, M. Perrier, A. Kamen

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Insights into the central metabolism of Spodoptera frugiperda (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5) insect cells by radiolabeling studies

C. Benslimane, C.B. Elias, J. Hawari, A. Kamen

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A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography

M.D.L.M. Segura, A. Kamen, P. Trudel, A. Garnier

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Production of adenoviral vectors [Production de vecteurs adénoviraux]

A. Kamen, I. Nadeau

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Micro-quantitation of lipids in serum-free cell culture media: A critical aspect is the minimization of interference from medium components and chemical reagents

C.F. Shen, J. Hawari, A. Kamen

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Insights into adenoviral vector production kinetics in acoustic filter-based perfusion cultures

O. Henry, E. Dormond, M. Perrier, A. Kamen

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High-performance liquid chromatographic total particles quantification of retroviral vectors pseudotyped with vesicular stomatitis virus-G glycoprotein

J. Transfiguracion, H. Coelho, A. Kamen

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Development and optimization of an adenovirus production process

A. Kamen, O. Henry

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Acoustic cell filter: A proven cell retention technology for perfusion of animal cell cultures

I.Z. Shirgaonkar, S. Lanthier, A. Kamen

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Size-exclusion chromatography purification of high-titer vesicular stomatitis virus G glycoprotein-pseudotyped retrovectors for cell and gene therapy applications

J. Transfiguracion, D.E. Jaalouk, K. Ghani, J. Galipeau, A. Kamen

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Production of adenovirus vector for gene therapy

I. Nadeau, A. Kamen

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Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency

P.L. Pham, S. Perret, H.C. Doan, B. Cass, G. St-Laurent, A. Kamen, Y. Durocher

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Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme

C.B. Elias, E. Carpentier, Y. Durocher, L. Bisson, R. Wagner, A. Kamen

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Quantitation of baculovirus particles by flow cytometry

C.F. Shen, J. Meghrous, A. Kamen

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Low-protein medium affects the 293SF central metabolism during growth and infection with adenovirus

I. Nadeau, P.A. Gilbert, D. Jacob, M. Perrier, A. Kamen

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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.

Y. Durocher, S. Perret, A. Kamen

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Validation of a high-performance liquid chromatographic assay for the quantification of adenovirus type 5 particles

J. Transfiguracion, A. Bernier, N. Arcand, P. Chahal, A. Kamen

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Monitoring motility, spreading, and mortality of adherent insect cells using an impedance sensor

J.H.T. Luong, M. Habibi-Rezaei, J. Meghrous, C. Xiao, K.B. Male, A. Kamen

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Increased production of active human β<inf>2</inf>-adrenergic/G<inf>αs</inf> fusion receptor in Sf-9 cells using nutrient limiting conditions

E. Carpentier, D. Lebesgue, A.A. Kamen, M. Hogue, M. Bouvier, Y. Durocher

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Improved high-performance liquid chromatographic method in the analysis of adenovirus particles

V. Klyushnichenko, A. Bernier, A. Kamen, E. Harmsen

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Dystrophin expression in muscle following gene transfer with a fully deleted ("Gutted") adenovirus is markedly improved by trans-acting adenoviral gene products

R. Gilbert, J. Nalbantoglu, G. Karpati, J.M. Howell, L. Davies, S. Fletcher, A. Amalfitano, B.J. Petrof, A. Kamen, B. Massie

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Understanding factors that limit the productivity of suspension-based perfusion cultures operated at high medium renewal rates

S. Mercille, M. Johnson, S. Lanthier, A.A. Kamen, B. Messie

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Production of Choristoneura fumiferana nucleopolyhedrovirus in C. fumiferana (CF-2C1 cells) in a 3 litre bioreactor using serum-free medium

J. Meghrous, A. Kamen, S.R. Palli, S.S. Sohi, G.F. Caputo, C. Bédard

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On-line monitoring of physiological parameters of insect cell cultures during the growth and infection process

A. Zeiser, C.B. Elias, R. Voyer, B. Jardin, A.A. Kamen

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On-line measurement of green fluorescent protein (GFP) fluorescence for the monitoring of recombinant adenovirus production

P.-A. Gilbert, A. Garnier, D. Jacob, A. Kamen

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Human 293 cell metabolism in low glutamine-supplied culture: Interpretation of metabolic changes through metabolic flux analysis

I. Nadeau, J. Sabatié, M. Koehl, M. Perrier, A. Kamen

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Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 107 cells per mL and production of β-galactosidase at high cell density by fed batch culture

C.B. Elias, A. Zeiser, C. Bédard, A.A. Kamen

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A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture

Y. Durocher, S. Perret, E. Thibaudeau, M.-H. Gaumond, A. Kamen, R. Stocco, M. Abramovitz

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Fed-batch culture of Sf-9 cells supports 3 x 107 cells per ml and improves baculovirus-expressed recombinant protein yields

C. Bédard, S. Perret, A.A. Kamen

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Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies

I. Nadeau, A. Garnier, J. Côté, B. Massie, C. Chavarie, A. Kamen

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Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells

A. Garnier, R. Voyer, R. Tom, S. Perret, B. Jardin, A. Kamen

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Scale-up of recombinant virus and protein production in stirred-tank reactors.

R.L. Tom, A.W. Caron, B. Massie, A.A. Kamen

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Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection

R.L. Tom, M.T. Debanne, C. Bédard, A.W. Caron, B. Massie, A.A. Kamen

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Determination of mass transfer parameters in surface aerated bioreactors with bubble entrainment

A.A. Kamen, A. Garnier, G. André, J. Archambault, C. Chavarie

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Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells

A. Garnier, J. Côté, I. Nadeau, A. Kamen, B. Massie

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Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures

C. Bédard, A. Kamen, R. Tom, B. Massie

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Growth, Nutrient Consumption, and End‐Product Accumulation in Sf‐9 and BTI‐EAA Insect Cell Cultures: Insights into Growth Limitation and Metabolism

C. Bédard, R. Tom, A. Kamen

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Design parameters and performance of a surface baffled helical ribbon impeller bioreactor for the culture of shear sensitive cells

A.A. Kamen, C. Chavarie, G. André, J. Archambault

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Culture of insect cells in helical ribbon impeller bioreactor

A.A. Kamen, R.L. Tom, A.W. Caron, C. Chavarie, B. Massie, J. Archambault

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