A. Kamen

Latest

• Extended gene expression for Gag VLP production achieved at bioreactor scale
• Advancements in the design and scalable production of viral gene transfer vectors
• Gene Transfer of ZMapp Antibodies Mediated by Recombinant Adeno-Associated Virus Protects Against Ebola Infections
• Accelerated mass production of influenza virus seed stocks in HEK-293 suspension cell cultures by reverse genetics
• Critical phases of viral production processes monitored by capacitance
• Manufacturing of recombinant adeno-associated viruses using mammalian expression platforms
• Process intensification for high yield production of influenza H1N1 Gag virus-like particles using an inducible HEK-293 stable cell line
• Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate
• Optimization and scale-up of cell culture and purification processes for production of an adenovirus-vectored tuberculosis vaccine candidate
• Influence of HEK293 metabolism on the production of viral vectors and vaccine
• Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures
• Development of a vectored vaccine against Hepatitis E virus
• Establishment and validation of new complementing cells for production of E1-deleted adenovirus vectors in serum-free suspension culture
• Evaluation of homogeneity and genetic stability of REOLYSIN® (pelareorep) by complete genome sequencing of reovirus after large scale production
• Real-time monitoring of influenza virus production kinetics in HEK293 cell cultures
• A novel polyethyleneimine-coated adenoassociated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: Preparation and in vitro analysis
• Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase, improves adenovirus production
• Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge
• Process optimization and scale-up for production of rabies vaccine live adenovirus vector (AdRG1.3)
• Cell Transfection
• Monitoring lentiviral vector production kinetics using online permittivity measurements
• Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII
• Unraveling the metabolism of HEK-293 cells using lactate isotopomer analysis
• An efficient process for the purification of helper-dependent adenoviral vector and removal of helper virus by iodixanol ultracentrifugation
• Effect of surface charge on the cellular uptake and cytotoxicity of fluorescent labeled cellulose nanocrystals
• New protocol for lentiviral vector mass production.
• Reassessing culture media and critical metabolites that affect adenovirus production
• Recent progress in lentiviral vector mass production
• An efficient and scalable process for helper-dependent adenoviral vector production using polyethylenimine-adenofection
• Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures
• Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media
• From the first to the third generation adenoviral vector: What parameters are governing the production yield?
• Identification of critical infection parameters to control helper-dependent adenoviral vector production
• Probing inhibitory effects of destruxins from Metarhizium anisopliae using insect cell based impedance spectroscopy: Inhibition vs chemical structure
• Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions
• Identification of host proteins associated with retroviral vector particles by proteomic analysis of highly purified vector preparations
• Probing inhibitory effects of Antrodia camphorata isolates using insect cell-based impedance spectroscopy: Inhibition vs chemical structure
• Purification of retrovirus particles using heparin affinity chromatography
• Rapid and reliable quantification of reovirus type 3 by high performance liquid chromatography during manufacturing of Reolysin®
• A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus
• Characterization of yeastolate fractions that promote insect cell growth and recombinant protein production
• Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography
• Generation of a high-titer packaging cell line for the production of retroviral vectors in suspension and serum-free media
• High yield purification of functional baculovirus vectors by size exclusion chromatography
• Monitoring the physiological state of mammalian cell perfusion processes by on-line estimation of intracellular fluxes
• On-line monitoring of cell size distribution in mammalian cell culture processes
• Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system
• Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification
• Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system
• Scalable serum-free production of recombinant adeno-associated virus type 2 by transfection of 293 suspension cells
• Validation of a high-performance liquid chromatographic assay for the quantification of Reovirus particles type 3
• Analysis of baculovirus aggregates using flow cytometry
• Downstream processing of oncoretroviral and lentiviral gene therapy vectors
• Large-scale transfection of mammalian cells for the fast production of recombinant protein
• Multivariable nonlinear control of biomass and metabolite concentrations in a high-cell-density perfusion bioreactor
• Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation
• Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor
• Stability of serum-free and purified baculovirus stocks under various storage conditions
• Use of cell bleed in a high cell density perfusion culture and multivariable control of biomass and metabolite concentrations
• A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography
• Insights into the central metabolism of Spodoptera frugiperda (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5) insect cells by radiolabeling studies
• Metabolic flux analysis of HEK-293 cells in perfusion cultures for the production of adenoviral vectors
• Production and formulation of adenovirus vectors
• Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme
• Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis
• Acoustic cell filter: A proven cell retention technology for perfusion of animal cell cultures
• Development and optimization of an adenovirus production process
• High-performance liquid chromatographic total particles quantification of retroviral vectors pseudotyped with vesicular stomatitis virus-G glycoprotein
• Insights into adenoviral vector production kinetics in acoustic filter-based perfusion cultures
• Micro-quantitation of lipids in serum-free cell culture media: A critical aspect is the minimization of interference from medium components and chemical reagents
• Production of adenoviral vectors [Production de vecteurs adénoviraux]
• Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme
• Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency
• Production of adenovirus vector for gene therapy
• Size-exclusion chromatography purification of high-titer vesicular stomatitis virus G glycoprotein-pseudotyped retrovectors for cell and gene therapy applications
• High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.
• Low-protein medium affects the 293SF central metabolism during growth and infection with adenovirus
• Quantitation of baculovirus particles by flow cytometry
• Dystrophin expression in muscle following gene transfer with a fully deleted ("Gutted") adenovirus is markedly improved by trans-acting adenoviral gene products
• Improved high-performance liquid chromatographic method in the analysis of adenovirus particles
• Monitoring motility, spreading, and mortality of adherent insect cells using an impedance sensor
• Validation of a high-performance liquid chromatographic assay for the quantification of adenovirus type 5 particles
• 293SF metabolic flux analysis during cell growth and infection with an adenoviral vector
• A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture
• Human 293 cell metabolism in low glutamine-supplied culture: Interpretation of metabolic changes through metabolic flux analysis
• On-line measurement of green fluorescent protein (GFP) fluorescence for the monitoring of recombinant adenovirus production
• Production of Choristoneura fumiferana nucleopolyhedrovirus in C. fumiferana (CF-2C1 cells) in a 3 litre bioreactor using serum-free medium
• Adaptive control at low glucose concentration of HEK-293 cell serum-free cultures
• Adenovirus-mediated utrophin gene transfer mitigates the dystrophic phenotype of mdx mouse muscles
• A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals
• Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells
• Study of adenovirus production in serum-free 293SF suspension culture by GFP-expression monitoring
• Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells
• Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies
• Insect cell density in bioreactor cultures can be estimated from on-line measurements of optical density
• Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures
• Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells
• Growth, Nutrient Consumption, and End‐Product Accumulation in Sf‐9 and BTI‐EAA Insect Cell Cultures: Insights into Growth Limitation and Metabolism